Organization Overview

The Proteomics Resource Center (PRC) at The Rockefeller University masters many aspects of liquid chromatography/mass spectrometry applied to metabolomics and proteomics. Examples include de novo sequencing, and quantitative analysis based on label-free quantitation as well as heavy isotope labeled samples. The PRC is equipped with Orbitrap-type mass spectrometers (high resolution/high mass accuracy) and can separate analytes using both nano and high-flow liquid chromatography. The Center is a source for help with planning of mass spectrometry-based experiments, and it has the capability to offer in-depth collaborative analysis. Also, the PRC is operated in a boutique style, encouraging the scientists of the Center to work closely with users to create a tailored approach to fit the many unique questions that can be answered by analytical mass spectrometry. The Center is equipped with multiple high-performance servers for data analysis.

Overview

We seek an enthusiastic, diligent and team-oriented colleague to join the Proteomics Platform in the Proteomics Resource Center (https://www.rockefeller.edu/proteomics/). The candidate is expected to support the community by engaging in proteomics research which includes providing input on experimental design, running experiments and disseminating results.

Responsibilities

Responsibilities include:

• Consulting with the Director to identify project goals, experimental design, and deadlines

• Interacting with team members in support of overall Center operations

• Complying with lab safety guidelines

• Tracking samples and submitting billing and purchasing information

• Maintaining lab records

• Generating detailed technical documents and sample operating procedures

• Maintaining laboratory appearance and monitoring chemical inventory and supplies

• Maintaining and operating LC-MS (Dionex 3000, EasyLC 1200, Vanquish Neo, Q-Exactive HF, Orbitrap Fusion and Orbitrap Ascend) and related equipment with a focus on high quality data generation

• Work with samples, from a variety of biological matrices, using various sample processing techniques

• Developing and implementing methodologies related to proteomics

• Processing, analyzing, and interpreting data using open source and vendor software

• Preparation of detailed reports for investigators

• Interacting with and advising investigators regarding study design and implementation

• Performing related duties and responsibilities as assigned/requested

Qualifications

Required:

• M.S. (with 3+ years of related work experience) required; Ph.D. (with emphasis in proteomics/ mass spectrometry/ analytical chemistry or related field) preferred

• In-depth experience with liquid chromatography coupled to mass spectrometry-based proteomics including method development

• Experience with high resolution mass spectrometers (preferably Orbitrap MS) including operation, calibration, tuning, cleaning, and other maintenance tasks.

• Strong understanding and hands-on experience with proteomics workflows including targeted, DDA, DIA and TMT applied to single protein analysis, affinity purifications, and global profiling, Expert competency in one or more of the following:

• Quantitative Proteomics Workflows: Absolute and relative quantification of peptides and proteins from biological matrices. Development and implementation of entire quantitative workflow (sample handling to data analysis)

• Processing of HRMS data with vendor and or open-source software

• Sample preparation of biological (e.g., plasma, tissue) and synthetic samples (e.g., standards) using a variety of sample processing techniques (e.g., liquid-liquid extraction, precipitation, solid-phase extraction)

• Strong communication (oral and written English) and interpersonal skills

• Strong organizational and multitasking skills

• Ability to work independently as well as part of a team

• Accurate and detailed record keeping in a lab notebook and or LIMS system

• Lab safety and general conduct

• Strong analytical and wet lab skills

• Ability to maintain and expand knowledge of mass spectrometry techniques through self-education, dialog with other experts, reading literature, and attending conferences and courses as necessary

• Practical experience with bioinformatics and statistical analysis is desirable

The Rockefeller University does not discriminate in employment on the basis of race, color, religion, sex (including pregnancy, gender identity), national origin, political affiliation, sexual orientation, marital status, disability, genetic information, age, membership in an employee organization, retaliation, parental status, military service or other non-merit factor. All qualified applicants will receive consideration for employment without regard to the characteristics listed above.

The salary of the finalist selected for this role will be set based on various factors, including but not limited to organizational budgets, qualifications, experience, education, licenses, specialty, and training. The hiring range provided represents The Rockefeller University's good faith and reasonable estimate of the range of possible compensation at the time of posting.

Compensation Range: Min

USD $70,000.00/Yr.

Compensation Range: Max

USD $100,000.00/Yr.