Research Technician

Seeking a research technician to work in a dynamic and fast-paced Translational Oncology research laboratory at the Hillman Cancer Center of the University of Pittsburgh Medical Center. The candidate will work under the direction of a physician-scientist in the Department of Radiation Oncology and will help carry out experiments investigating pediatric brain tumor cancer biology and the response to DNA damage agents. Roles will include cell culture, in vitro cell viability assays, Western Blot, DNA/RNA extraction, mouse work including orthotopic tumor implantation, treating tumor-bearing mice (radiation, drug administration), monitoring tumor growth, and harvesting mouse tissues for further analyses. Experience doing basic science research and cell culture is required.

Candidates should have at least a Bachelors of Science in biology, biochemistry, neuroscience, bioengineering, biomedical engineering, and one year of post-graduate research experience.

Key Responsibilities:

• Maintain an organized and complete lab notebook
• propagate cells in cell cultures and perform in vitro viability assays and clonogenic assays
• perform western blots to characterize cell lines, tumor tissues, and mouse tissues
• Extract DNA and RNA from mouse tumor tissue
• Orthotopic injection of tumor cell lines in mice
• In-vivo bioluminescence imaging to track tumor growth
• Collecting tumor tissue from mice
• Perform staining and flow cytometry analysis on murine and human cells
• treatment of tumor bearing mice with radiation using both external beam and/or radiopharmaceuticals
• Meet or communicate by e-mail with PI to plan experiments, troubleshoot problems, and discuss results
• Assist in recruiting
• Maintain reagents or equipment in the lab
• Assist in the development and optimization of new research procedures Job Summary

Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.

Essential Functions

Strong organizational skills and attention to detail is required. Must have the ability to work independently as well as part of a collaborative team. Must have excellent communication skills and the ability to maintain meticulous records.

Physical Effort

Moderate physical effort Moderate physical effort required. May be required to stand/move for several hours at a time. May be required to move objects up to 50 pounds with or without assistance of equipment.

The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.

Assignment Category: Full-time regular Job Classification: Staff.Laboratory Research Technician II Job Family: Research Job Sub Family: Laboratory Campus: Pittsburgh Minimum Education Level Required: Bachelors Degree Minimum Years of Experience Required: 1 Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement. Work Schedule: Monday - Friday Work Arrangement: Monday - Friday Hiring Range: To be determined based on qualifications Relocation_Offered: No Visa Sponsorship Provided: No Background Check: For position finalists, employment with the U iversity will require successful completion of a background check Child Protection Clearances: Not Applicable Required Documents: Resume Optional Documents: Cover Letter PI245268191