As a community, the University of Rochester is defined by a deep commitment to Meliora - Ever Better. Embedded in that ideal are the values we share: equity, leadership, integrity, openness, respect, and accountability. Together, we will set the highest standards for how we treat each other to ensure our community is welcoming to all and is a place where all can thrive.

Job Location (Full Address):

Select a Shipping Address, Rochester, New York, United States of America, 14627

Opening:

Worker Subtype:

Regular

Time Type:

Full time

Scheduled Weekly Hours:

40

Department:

400058 Medicine M&D-Nephrology Div

Grade:

UR URG 105 H

Compensation Range:

$18.89 - $26.44

The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.

Responsibilities:

Performs complex laboratory assignments, planning and conducting experiments of limited scope or a portion of a larger and more diverse project. Leads tasks associated with research activities and lab support for lower-level technicians. Serves as unit operations liaison to ensure compliance with departmental or University policies, procedures, and defined internal controls. Ensures accountability and stewardship of University resources (operational, financial, and human) in compliance with departmental standards and proceduresSchedule 8 AM-4:30 PM Responsibilities GENERAL PURPOSE: Under some supervision and with latitude for exercising independent technical judgment, is responsible for carrying out complex research procedures related to protein purification, DNA cloning, mammalian and insect cell culture, and performing Luminex assays to test human and rodent sera. Plans, develops and conducts new experimental protocols. Additional work with rodent colonies, including vaccination, intransal influenza infection, euthanasia, organ dissection and primary B and T cell isolation and culture. Ability to perform and co-ordinate multiple tasks on a daily basis. Will also ensure maintenance of laboratory supplies, equipment and proper disposal of all materials. SPECIFIC DUTIES INCLUDE: - Molecular biology work focusing on influenza and corona virology, such as gene cloning of Influenza hemagglutinin (HA) proteins, spike protein (S) of coronaviruses and HA, S protein expression and purification using baculovirus expression systems, including maintenance and infection of insect cell lines. DNA isolation using commercial maxi- and mini-prep kits. PCR amplification and sub cloning of wild type and mutant HA gene sequences into baculovirus expression vectors. May also include the culture and production of influenza viruses, and performing influenza hemagglutination (HAI) and microneutralization (MN) assays. - Isolation and storage of human and mouse serum for serological analysis; performing luminex based assays assessing antibody levels to influenza HA and coronavirus S proteins. - Independently documenting experimental protocols, as well as adapting these protocols to meet the needs of individual experiments and special situations. Collecting and analyzing scientific data. Maintaining technical expertise and awareness of trends through professional journals and research team meetings. Performing literature searches for material relevant to research projects - Operating and maintaining complex experimental equipment including Luminex and ELISA readers, spectrophotometers, PCR machines, and the like. - Involvement in mouse studies, such as assisting in the maintenance of rodent colonies, while also performing intranasal influenza infections, blood collection through submandibular bleeds and terminal exsanguination, i.m. and i.p. injections, euthanasia and spleen/lymph node harvesting. - Assist in ordering laboratory supplies as well as maintaining an accurate electronic inventory of laboratory supplies, chemicals and reagents. Other duties as assigned. REQUIREMENTS: - Associate's degree in appropriate discipline required. - Degree in science or engineering discipline preferred - Bachelor's degree preferred - 4-5 years specialized experience in related field, or equivalent combination of education and experience required. - Molecular experience including DNA cloning and purification, PCR, protein purification and mouse work highly desirable. Experience with tissue culture, and multiplex assays desirable. Computer skills with Excel and Adobe graphics software, and/or bioinformatics experience also desirable The University of Rochester is committed to fostering, cultivating, and preserving a culture of equity, diversity, and inclusion to advance the University's mission to Learn, Discover, Heal, Create - and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion/creed, sex, sexual orientation, citizenship status, or any other status protected by law. This commitment extends to the administration of our policies, admissions, employment, access, and recruitment of candidates from underrepresented populations, veterans, and persons with disabilities consistent with these values and government contractor Affirmative Action obligations.

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Learn. Discover. Heal. Create.

Located in western New York, Rochester is our namesake and our home. One of the world’s leading research universities, Rochester has a long tradition of breaking boundaries—always pushing and questioning, learning and unlearning. We transform ideas into enterprises that create value and make the world ever better.

If you’re looking for a career in higher education or health care, the University of Rochester may offer the perfect opportunity for your background and goals

At the University of Rochester, we commit to diversity, equity, and inclusion and united by a strong commitment to be ever better—Meliora. It is an ideal that informs our shared mission to ensure all members of our community feel safe, respected, included, and valued.