Req #: 241548

Department: DEPARTMENT OF PEDIATRICS

Appointing Department Web Address: https://www.seattlechildrens.org/research/

Posting Date: 12/16/2024

Closing Info: Open Until Filled

Salary: $22.43 - $44.80 per hour

Shift: First Shift

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701_a11y.pdf)

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty.

The UW Department of Pediatrics is dedicated to improving the health of all children by educating physicians of the future, advancing research, advocating for children, and providing the nation's best pediatric clinical care. The Division of Rheumatology within the Department of Pediatrics cares for children with rheumatic diseases, autoimmune and autoinflammatory diseases that affect the joints or multiple body systems. The Division is composed of 13 faculty members with a diverse set of research interests and expertise. Dr. Canny is a pediatric rheumatologist with a focus on basic and translational studies of hyperinflammatory syndromes that affect children. She seeks a dedicated and enthusiastic Temporary Research Scientist/Engineer 1 with interests in immunology, cellular biology, and an understanding of human disease. The goals of the research program are to understand the role of inflammatory molecules (cytokines and ligands for innate immune sensors) in triggering monocyte responses and shaping T cell responses during hyperinflammatory syndromes. Laboratory work will be conducted under Dr. Canny’s supervision at the Benaroya Research Institute on First Hill.

Job duties include but are not limited to: Bench Research - Engage in laboratory work in support of funded research projects o Tissue culture: maintain sterile technique and isolate cells from human blood samples, culture and activate human immune cells. o Basic molecular biology techniques: nucleic acid isolation, reverse transcription, quantitative PCR. o Immunology techniques: flow cytometry, ELISA and other established techniques. Sample handling and management - Researcher will ensure that samples are inventoried and stored properly. o Sample intake and processing: Label, track, process (isolate DNA or RNA, plasma or serum, cells) and store human samples (blood, cells, plasma/serum, DNA, RNA, cell lines, tissue) in compliance with human subject research regulations. General research support - Researcher will help with overall operations of the laboratory as assigned by the lab manager or PI. o Perform other research duties as required such as: protocol implementation and trouble shooting. o Data Presentation: summarize research results in presentations for lab meetings as well as for use in public presentations and publications. o Maintain appropriate research documentation and databases.

Minimum Qualifications:

• Bachelor’s degree in Biology, Cellular or Molecular Biology, or related field. • 2-4 Months applicable work experience. • Coursework or equivalent research experience in immunology. • Excellent attention to detail and strong organizational skills. • Able to work independently and as part of a multidisciplinary team. • Demonstrated practical experience and proficiency in basic molecular biology procedures including spectrophotometry for measuring nucleic acid concentration, PCR primer design, PCR amplification, gel electrophoresis, RNA isolation. • Experience handling human blood samples and cells or extensive experience with mammalian cell culture. • Knowledge of and/or experience with flow cytometry and other immunology techniques. • Ability to communicate experimental results clearly using strong computer skills in Word, Excel and PowerPoint.

Desirable Qualifications:

• Experience with flow cytometry including sample preparation and analysis. • Knowledge of CRISPR-Cas9 editing. • Familiarity with coding using R. • Experience isolating immune cells from blood. • Comfort with extracting and handling RNA.

The employee will receive training in human subjects’ research and will be required to demonstrate knowledge and understanding of, and maintain confidentiality of patient privacy rights, employee information, Department of Pediatrics strategic plans and initiatives, financial information or other sensitive materials and information in printed, electronic or verbal form, which may jeopardize employee rights, the organization’s operations or privacy of patients. They will access and use the minimum necessary information to perform job responsibilities and duties and only for authorized purposes.

Application Process:

The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter, and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.